Dr. Sergio Chai, Ph.D. - High Throughput Screening Specialist

Dr. Sergio Chai Ph.D. High Throughput Screening Specialist at St. Jude Children's Research Hospital. University of Massachusetts Amherst. Memphis, United States. Expertise in high throughput screening: assay development (biochemical and cell-based), validation, implementation, robotics/laboratory automation operation, large-scale data analysis, troubleshooting and screening campaign management. Comprehensive experience in drug discovery with emphasis in chemical biology, biochemistry and analytical chemistry. Involved in every stage of early drug discovery process, including target validation, hit identification and lead optimization. In-depth knowledge of biochemical characterization of HTS hits/leads including enzyme kinetics, binding studies, SAR and selectivity profiling. Expertise in mining, analyzing and visualizing data, and customizing programs. Experience in structure-based drug discovery (crystal structure analysis and docking). Project leader of multi-disciplinary teams, collaborating with medicinal chemists, biologists, computational and structural scientists. Over 30 publications in protein characterization/inhibition and HTS technologies.

TECHNOLOGIES:

Assay readouts: luminescence, fluorescence intensity (FI), fluorescence polarization (FP), TR-FRET, absorbance, mass spectrometry. HTS instrumentation: plate readers, automated liquid dispensers, semi-automated and full-deck robotics. Chromatography: protein purification systems, HPLC, gel electrophoresis. Computer literacy: familiarity with various software applications, and scripting in pipeline pilot and VBA.